GT-Digital NPM1-W288fs*12 Insertion Detection Assay

Featured Product

GT-Digital NPM1-W288fs*12 Insertion Detection Assay for the Bio-Rad QX200™ Droplet Digital™ PCR System

The dPCR NPM1 Mutation Detection Assay Kit for the Bio-Rad QX200™ Droplet Digital™ PCR System is a Research Use Only molecular reagent kit containing all primers, probes, and controls for the detection of DNA with NPM1 mutations common in acute myeloid leukemia (AML). The assay is designed to detect over 40 mutation types, including Type A, B, and D mutations.

This drop-off assay binds within the amplicon downstream from the c.863 insertion site and to a non-mutated sequence at the c.863 insertion site resulting in a drop-off assay with the presence of an insertion mutation and a control that detects all copies of amplicon. The reaction provides detection of insertion mutations at W288 from the non-mutated sequences. The reaction does not discriminate between the types of insertions at W288. This kit includes one primer probe solution and qualitative controls. The W288cfs*12 control contains the most common insertion (Type A: c.863insTCTG).

For research use only. Not intended for diagnostic use.

GT-Digital NPM1-W288fs*12 Insertion Detection Assay

Reaction Mutation Targets

Reaction is for detection of insertion mutations at W288 from the non-mutated sequences

Controls Included

This kit includes three controls including a qualitative NPM1 wild-type, Type A mutation, and an NPM1 Type A: Wild-Type Mixed Positive controls.

The W288cfs*12 mutation control contains the most common insertion (Type A: c.863insTCTG)

Highly Sensitive
Detect as few as 3 target copies per reaction

Robust
Eliminate interference from PCR inhibitors compared to real-time quantitative PCR (RT-qPCR)

High Throughput
Multiplex for faster throughput and lower reagent cost

Compatible
Use with formalin-fixed paraffin-embedded (FFPE), liquid biopsy, fresh/frozen tissue, cell-free DNA (cfDNA), circulating tumor (ctDNA), bone marrow, whole blood, and low-yield samples

Our NPM1 Oncology Assays Are Optimized for the Bio-Rad QX200™ Droplet Digital™ PCR System with More in Development!

Bio-Rad QX200™ ddPCR™ System

Key Benefits

Sensitive

Detect as few as 3 copies of the NPM1 gene targets per reaction and genotype samples with low nucleic acid concentrations

Multiplexed

Leverage our highly sensitive technology to amplify low concentration targets in each assay, saving you time and money

Easy to Use

Assay your samples with validated kits that include all necessary primers, probes, and controls

Robust

Minimize interference from PCR inhibitors using dPCR technology

Popular Oncology Research Kits

Quantify Your Target in 3 Easy Steps

1
Receive

Receive kit via overnight shipping within five business days

2
Process

Extract nucleic acid from your sample using your lab’s preferred method

3
Assay

Perform the PCR assay on your sample using your instrument of choice

GT Molecular dPCR Assay Advantages

Increased Sensitivity
Detect low DNA concentrations with greater sensitivity than RT-qPCR
Multiplex Technology
Assay several targets in each dPCR reaction, saving you time and money
Decreased Inhibitor Interference
Small reactions dilute the effects of any PCR inhibitors present in a sample

FAQs

Don’t See Your Target?

Let GTM Customize an Assay for You

Learn More