Microbial Source Tracking
Identify sources of pollution and disease spread using our microbial source tracking (MST) assay kits and testing services. Quickly get the insight you need to pinpoint fecal contamination, apply proper mitigation steps, prevent future health risks, and decrease negative economic or reputational impacts. We are an industry leader in developing assays and developing processing protocols for water and wastewater samples that provide the best results possible.
Contact us for use with other different sample types and platforms available for RT-PCR and digital PCR platforms such as the Bio-Rad QX200™/QX600™, QIAGEN QIAcuity®, Roche Digital LightCycler® platforms.
GT Molecular’s GT-Digital Microbial Source Tracking Panel v1.0 for the QIAGEN QIAcuity® Digital PCR Platform
- 5-plex assay that targets key sources of fecal contamination for microbial source tracking (MST)
- Comprehensive Detection – Detect human, cow, gull, and dog-associated Bacteroides species, plus E. coli for complete microbial source tracking.
- High Sensitivity & Specificity – Uses validated markers (HF183, CowM2, Gull4, BacCan, EC23S) for accurate contamination identification.
- Streamlined Workflow – Includes all primers, probes, and controls needed for robust, multiplexed detection of all targets in a single well!
- Validated for use in recreational and environmental water samples. Contact us for applications with other sample types and available platforms.
Trace Pollution Origin
Bacteria found in different host organisms are identified by targeting known gene differences. Determine points of fecal contamination within a watershed or surface water to get the actionable insight needed. Pollution sources and example targets include:
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E. coli EC23S target
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Human (HF183)
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Cow (CowM2)
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Pig (Pig2Bac)
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Gull (Gull4)
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Dog (BacCan)
Please contact us for more available targets.
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Rapid DNA Testing
Unlike culture-based methods that require overnight growth and only show the presence/absence of fecal contamination, PCR testing provides rapid same day results that identify different sources of fecal contamination (i.e. human, cow, dog, etc.). More rapid testing allows for faster response and decision making to mitigate, minimize, and prevent infection, disease spread and further economic impacts.
Ensure Treatment Compliance and Improve Water Quality
Whether you want to improve, problem solve for compliance, or maintain your wastewater treatment process, use our assay kits or comprehensive wastewater testing service to track treatment efficiency and stay on top of variations in treatment processes. This method can be used in combination with our 16S microbial sequencing services that includes relative quantification, classification of microbial communities, process-relevant insights and functional group analysis to get the results you need for more reliable treatment and gaining a deeper understanding of your system.
End-to-End Testing Solution
Don’t have the staff or capacity to do the testing yourself? Simplify your testing using our complete service from sample collection to reporting with our included sample collection kit, shipping, preparation, analysis, and reporting. You send us the sample using the provided sample kit with prepaid shipping and we send you results fast so you can make informed decisions when you need it most.
MST Assays for the Bio-Rad QX200™/QX600™ ddPCR™ system
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Duplexed assays that target key sources of fecal contamination for microbial source tracking (MST)
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Kits include controls and step-by-step data analysis guidance
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Validated for use in recreational and environmental water samples. Contact us for applications with other sample types and available platforms.
Quantify Sources of Fecal Contamination
Use digital PCR to detect and quantify sources of fecal contamination. Digital PCR has the advantage over traditional RT-qPCR offerings by providing absolute quantification and lower sample inhibition from tough matrices such as those found in environmental water and wastewater samples.
Quantify Your Targets in 3 Easy Steps
Receive kit via overnight shipping within five business days
Extract nucleic acid from your sample using your lab’s preferred method
Perform the PCR assay on your sample using your instrument of choice
Most Requested Products
GT-Digital Microbial Source Tracking (MST) Human;Cow Assay v1.0 for the Bio-Rad QX200™ or QX600™ Droplet Digital™ PCR System is a molecular reagent kit consisting of all primers, probes, and controls necessary for detection of microbial DNA specific to human and cow Bacteroides species.
GT-Digital Microbial Source Tracking Human;E.coli;Cow;Gull;Dog Panel v1.0 for the QIAGEN QIAcuity® Digital PCR System is a molecular reagent kit consisting of all primers, probes, and controls necessary for detection of microbial DNA specific to human, cow, gull and dog Bacteroides species, as well as E. coli species.