GT-Digital IDH1/IDH2/NPM1 Mutation Panel

GT-Plex™ IDH1/IDH2/NPM1 Mutation Panel

Quantify common mutations found in human IDH1, IDH2, and NPM1 genes (RUO) all of which are associated with acute myeloid leukemia (AML). Isocitrate dehydrogenase 1 and 2 (IDH1/2) mutations are genetic changes that affect enzymes involved in cellular metabolism and DNA repair. These mutations are frequently found in several types of cancer, including gliomas, chondrosarcomas, and hematologic malignancies. The nucleophosmin 1 gene (NPM1) encodes a nucleocytoplasmic shuttling protein with prominent nucleolar localization. NPM1 mutations are among the most common abnormalities in AML.

Research Use Only (RUO). Not intended for diagnostic purposes. Contact us for assay kit availability on more platforms!

GT-Digital IDH1/IDH2/NPM1 Mutation Panel v1.0 for the Roche Digital LightCycler® System

Highly multiplexed assay measures six IDH1/IDH2/NPM1 targets in a single well
Patent-pending technology allows for highly specific detection and quantification
Kits include controls and step-by-step data analysis guidance
Assay detects key IDH1/IDH2/NPM1 mutations in ctDNA even in very low concentration plasma samples

For research use only. Not intended for diagnostic use.

GT-Plex™ Multiplexed IDH1/IDH2/NPM1 Panel

IDH1/IDH2/NPM1 Targets in One Assay Solution

IDH1-R132 (control region)
IDH2-R172K
IDH1-R132C/H
IDH2-R140Q
NPM1-uni (control region)
NPM1-W288 insertions

Controls Included
Assay includes three control solutions including an IDH1/IDH2/NPM1 non-mutant qualitative positive control (0% VAF), mutant qualitative positive control (100% VAF), and a mixed qualitative positive control (10% VAF) for all target regions.

Highly Sensitive
Detect as few as 3 target copies per reaction

Robust
Eliminate interference from PCR inhibitors compared to real-time quantitative PCR (RT-qPCR)

High Throughput
Multiplex for faster throughput and lower reagent cost

Compatible
Use with DNA from formalin-fixed paraffin-embedded (FFPE) samples, fresh/frozen tissue, whole blood, cell-free DNA (cfDNA), and circulating tumor DNA (ctDNA)

Our IDH1/IDH2/NPM1 Oncology Assays Are Optimized for the Roche Digital PCR Platform with More in Development!

Roche Digital LightCycler® System

Key Benefits

Sensitive

Detect as few as 3 copies of the IDH1/IDH2/NPM1 gene targets per reaction and genotype samples with low nucleic acid concentrations

Multiplexed

Leverage our GT-Plex™ technology to amplify more targets in each assay, saving you time and money

Easy to Use

Assay your samples with validated kits that include all necessary primers, probes, and controls

Robust

Minimize interference from PCR inhibitors using dPCR technology

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GT-Digital IDH1/IDH2/NPM1 Mutation Panel v1.0 for the Roche Digital LightCycler™ System

(Cat# 100730) The GT-Digital IDH1/IDH2/NPM1 Mutation Panel for the Roche Digital LightCycler™ System is a Research-Use Only (RUO), patent-pending molecular reagent kit containing all primers, probes, and controls for the detection and relative abundance of common mutations found in the human IDH1, IDH2, and NPM1 genes. This multi-target kit contains one assay solution and three […]

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Quantify Your Target in 3 Easy Steps

Receive

Receive kit via overnight shipping within five business days

Process

Extract nucleic acid from your sample using your lab’s preferred method

Assay

Perform the PCR assay on your sample using your instrument of choice

GT Molecular dPCR Assay Advantages

Increased Sensitivity

Detect low target DNA concentrations with greater sensitivity than RT-qPCR

Multiplex Technology

Assay several targets in each dPCR reaction, saving you time and money

Decreased Inhibitor Interference

Small reactions dilute the effects of any PCR inhibitors present in a sample

FAQs

What instruments can I use with the GT-Plex™ Multiplex dPCR Kits?

GT-Plex™ Multiplex dPCR Kits are optimized for each platform. Currently this assay is available on the Roche Roche Digital LightCycler® Platform. Contact us for availability on our other platform options such as the QIAGEN QIAcuity®, and Bio-Rad QX100™, QX200™, and QX600™ dPCR platforms.

Do you offer technical support?

Yes! GT Molecular’s technical support scientists are available from 8:00am to 5:00pm (MT) Monday through Friday to answer any questions regarding our products. Contact us at 1 970-498-1698 or through this form, and we’ll be happy to walk you through the workflow and answer any questions you may have.

Which targets are measured by the GT-Plex™ IDH1/IDH2/NPM1 Multiplex PCR Kit?

The GT-Digital IDH1/IDH2/NPM1 Panel targets non-mutated sequence IDH1-R132 and IDH2-R172K, IDH1-R132C/H, IDH2-R140Q mutations as well as NPM1-uni (control region in the same amplicon) and NPM1-W288 non-mutated sequence where common insertions occur resulting in a loss of signal that can be measured against the control signal in a drop-off reaction.

Contains enough material for 50 reactions.

What positive controls are used with the GT-Plex™ IDH1/IDH2/NPM1 Multiplex PCR Kit?

To ensure precision and reliability, the kit provides one assay solution and three qualitative controls. A positive control containing IDH1-R132H/C, IDH2-R172K, IDH2-R140Q, and NPM1-W288fs*12 control at 100% variant allele frequency (VAF), a mutated control at 10% VAF, and a non-mutated control at 0% VAF for all target regions.

How soon after placing an order can I expect delivery of the kit?

Once an order is placed, most assay kits are shipped within 5 business days on ice via FedEx Priority Overnight.

What sample types can be used with the GT-Digital IDH1/IDH2/NPM1 Multiplex PCR Kit?

Our oncology research kits are suitable for use with formalin-fixed paraffin-embedded (FFPE) tissues, liquid biopsy, fresh/frozen tissue, cell-free DNA (cfDNA), circulating tumor DNA (ctDNA), and low-yield samples. Our dPCR assays also tolerate samples that contain PCR inhibitors.