GT-Digital NPM1-W288fs*12 Insertion Detection Assay
Assay for the detection of over 40 NPM1-W288fs*12 mutations, including Type A, B, and D mutations. NPM1 is the most common mutation associated with Acute Myeloid Leukemia (AML) and this assay covers several of the most common of these mutation types.
Research Use Only (RUO). Not intended for diagnostic purposes. Contact us for assay kit availability on more platforms!
GT-Digital NPM1-W288fs*12 Insertion Detection Assay for the Bio-Rad QX200™ Droplet Digital™ PCR System
The dPCR NPM1 Mutation Detection Assay Kit for the Bio-Rad QX200™ Droplet Digital™ PCR System is a Research Use Only molecular reagent kit containing all primers, probes, and controls for the detection of DNA with NPM1 mutations common in acute myeloid leukemia (AML). The assay is designed to detect over 40 mutation types, including Type A, B, and D mutations.
This drop-off assay binds within the amplicon downstream from the c.863 insertion site and to a non-mutated sequence at the c.863 insertion site resulting in a drop-off assay with the presence of an insertion mutation and a control that detects all copies of amplicon. The reaction provides detection of insertion mutations at W288 from the non-mutated sequences. The reaction does not discriminate between the types of insertions at W288. This kit includes one primer probe solution and qualitative controls. The W288cfs*12 control contains the most common insertion (Type A: c.863insTCTG).
GT-Digital NPM1-W288fs*12 Insertion Detection Assay
Reaction Mutation Targets
Reaction is for detection of insertion mutations at W288 from the non-mutated sequences
Controls Included
This kit includes three controls including a qualitative NPM1 wild-type, Type A mutation, and an NPM1 Type A: Wild-Type Mixed Positive controls.
The W288cfs*12 mutation control contains the most common insertion (Type A: c.863insTCTG)
Highly Sensitive
Detect as few as 3 target copies per reaction
Robust
Eliminate interference from PCR inhibitors compared to real-time quantitative PCR (RT-qPCR)
High Throughput
Multiplex for faster throughput and lower reagent cost
Compatible
Use with formalin-fixed paraffin-embedded (FFPE), liquid biopsy, fresh/frozen tissue, cell-free DNA (cfDNA), circulating tumor (ctDNA), bone marrow, whole blood, and low-yield samples
Our NPM1 Oncology Assays Are Optimized for the Bio-Rad QX200™ Droplet Digital™ PCR System with More in Development!
Key Benefits
Detect as few as 3 copies of the NPM1 gene targets per reaction and genotype samples with low nucleic acid concentrations
Leverage our highly sensitive technology to amplify low concentration targets in each assay, saving you time and money
Assay your samples with validated kits that include all necessary primers, probes, and controls
Minimize interference from PCR inhibitors using dPCR technology
Popular Oncology Research Kits
Quantify Your Target in 3 Easy Steps
Receive kit via overnight shipping within five business days
Extract nucleic acid from your sample using your lab’s preferred method
Perform the PCR assay on your sample using your instrument of choice
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