GT-Plex™ EGFR Panels
Identify 30 actionable mutations in exons 18-21 of the EFGR gene using our patent-pending multiplexed digital PCR (dPCR) technology. Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that plays a key role in mediating cell signaling pathways. Activating mutations within EGFR exons 18-21, which encode a portion of the enzyme’s tyrosine kinase domain, are present in 10-50% of lung cancer cases and result in downstream signaling pathway hyperactivation, promoting cell survival. Tyrosine kinase inhibitor (TKI) therapy has shown remarkable efficacy in cancer patients harboring EGFR-mutations, highlighting the importance of identifying EGFR mutations in a timely manner to initiate appropriate treatment. TKIs halt overactive EGFR by competing with ATP for binding to the catalytic site of the enzyme, leading to attenuated kinase activity. TKI-resistance commonly occurs in initial responders, however, through secondary EGFR variants, such as T790M, that hinder TKI binding. Continuous monitoring can be used to assess for the emergence of TKI-resistant EGFR mutations. Request a copy of our application note to learn more!
Research Use Only (RUO)
GT-Plex™ Multiplexed EGFR Kit
EGFR Mutation Panel
Identify G719A/C/S, S768I, T790M, C797S, Ex20 A763-Y764insFQEA, L858R, L861Q, Ex19del, E746-P753 deletions
TKI Targets
Validated for 11 TKI targets, including 8 TKI-sensitive and 3 TKI-resistant mutations
Highly Sensitive
Detect as few as 3 target copies per reaction
Robust
Eliminate interference from PCR inhibitors compared to real-time quantitative PCR (RT-qPCR)
High Throughput
Multiplex for faster throughput and lower reagent cost
Compatible
Use with formalin-fixed paraffin-embedded (FFPE), liquid biopsy, fresh/frozen tissue, cell-free DNA (cfDNA), circulating tumor (ctDNA), and low-yield samples
GT-Plex™ Multiplex Digital PCR Assays Show Robust Performance Across a Broad Linear Dynamic Range
Our EGFR Oncology Assays Are Optimized for the Bio-Rad Digital PCR Platform. Contact us for rapid implementation on other platforms!
Key Benefits
Detect as few as 3 copies of the EFGR gene per reaction and genotype samples with low nucleic acid concentrations
Leverage our GT-Plex™ technology to amplify more targets in each assay, saving you time and money
Assay your samples with validated kits that include all necessary primers, probes, and controls
Minimize interference from PCR inhibitors using dPCR technology
Quantify Your Target in 3 Easy Steps
Receive kit via overnight shipping within five business days
Extract nucleic acid from your sample using your lab’s preferred method
Perform the PCR assay on your sample using your instrument of choice
Popular Oncology Research Kits
GT Molecular dPCR Assay Advantages
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