GT-Plex™ KRAS Panels

Assess KRAS G12C mutation and G12 allele status in a single multiplexed RT-PCR or digital PCR (dPCR) reaction. KRAS, a member of the RAS family, regulates signaling pathways responsible for cell proliferation, differentiation, and survival. KRAS is among the most frequently mutated oncogenes in human cancer, and mutations in KRAS can result in continuous cellular proliferation and cancer development. KRAS G12C derives from a single point mutation at codon 12 and is an oncogenic driver mutation that occurs in about 13% of non small-cell lung cancer (NSCLC) and 1%-3% of colorectal and other solid tumors. This substitution favors the activated state of KRAS, amplifying signaling pathways that lead to oncogenesis. KRAS G12C serves as a viable target for minimal residual disease (MRD) monitoring, as it predicts disease relapse. Request a copy of our application not to learn more!

Research Use Only (RUO)

GT-Plex™ Multiplexed KRAS G12C Kit

KRAS Mutation G12C

Identify the KRAS G12C mutation and G12 allele in a single reaction

Highly Sensitive

Detect as few as 3 target copies per reaction

Robust

Eliminate interference from PCR inhibitors compared to real-time quantitative PCR (RT-qPCR)

High Throughput

Multiplex for faster throughput and lower reagent cost

Compatible

Use with formalin-fixed paraffin-embedded (FFPE), liquid biopsy, fresh/frozen tissue, cell-free DNA (cfDNA), circulating tumor (ctDNA), and low-yield samples

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Available Kits

GT-Digital KRAS G12-G13 Mutation Typing Kit v1.0 for the QIAcuity™

(Cat# 100720) The GT-Digital KRAS G12-G12 Mutation Typing Kit for the QIAcuity™ Digital PCR System is a Research-Use Only (RUO) molecular reagent kit containing all primers, probes, and controls for the detection and quantification of the following KRAS mutations: G12C, G12V, G12S, G12A, G12R, G12D, G13C, and G13D. This assay does not recognize other KRAS […]

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GT Real-Time KRAS G12-G13 Typing PCR Kit

(Cat# 100615) The GT Real-Time KRAS G12-G13 Typing PCR Kit is a Research-Use Only (RUO) patent-pending molecular reagent kit containing all primers, probes, and controls necessary for the detection and discrimination of eight different mutations found within the G12 and G13 codon regions of the corresponding KRAS gene. The kit contains three separate assays for […]

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dPCR KRAS G12C;G12 Assay Kit for the Bio-Rad QX200®

(Cat# 100410) The dPCR KRAS G12C;G12 Assay Kit for the Bio-Rad QX200® Droplet Digital® PCR System is a Research-Use Only (RUO) molecular reagent kit containing all primers, probes, and controls for the detection and relative abundance of the G12C mutation found in the human KRAS gene. This duplexed assay contains a FAM probe that targets […]

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All Available KRAS Kits

GT-Plex™ Multiplex Digital PCR Assays Show Robust Performance Across a Broad Linear Dynamic Range

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Our KRAS Oncology Assays Are Optimized for QIAGEN, Bio-Rad, and Roche Digital PCR Platforms

QIAGEN QIAcuity® Digital PCR System

Bio-Rad QX200™ & QX600™ Droplet Digital PCR System

Roche Digital LightCycler®

Bio-Rad CFX96™, Opus™, and Others

Key Benefits

Sensitive

Detect as few as 3 copies of the KRAS gene per reaction and genotype samples with low nucleic acid concentrations

Multiplexed

Leverage our GT-Plex™ technology to amplify more targets in each assay, saving you time and money

Easy to Use

Assay your samples with validated kits that include all necessary primers, probes, and controls

Robust

Eliminate interference from PCR inhibitors using dPCR technology

Quantify Your Target in 3 Easy Steps

Receive

Receive kit via overnight shipping within five business days

Process

Extract nucleic acid from your sample using your lab’s preferred method

Assay

Perform the PCR assay on your sample using your instrument of choice

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GT-Digital IDH1/IDH2/NPM1 Mutation Panel v1.0 for the Roche Digital LightCycler™ System

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GT-Digital BCR-ABL Fusion Detection Panel v1.0 for the Roche Digital LightCycler™ System

(Cat# 100815) A Research-Use Only (RUO), patent-pending kit provides all the necessary primers, probes, and controls to detect and quantify human RNA with the following BCR-ABL translocations: major (e13a2, e13a3, e14a2, e14a3), minor (e1a2, e1a3), micro (e19a2, e19a3), and nano (e6a2, e6a3). This multi-target kit contains one assay solution, an ABL1 positive control, and positive […]

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GT Molecular dPCR Assay Advantages

Increased Sensitivity

Detect low-copy number transcripts with greater sensitivity than RT-qPCR

Multiplex Technology

Assay several targets in each dPCR reaction, saving you time and money

Decreased Inhibitor Interference

Small reactions dilute the effects of any PCR inhibitors present in a sample

FAQs

What instruments can I use with the GT-Plex™ Multiplex KRAS G12C PCR Kit?

Our GT-Plex™ Multiplex KRAS G12C PCR Kit is compatible with the QIAGEN QIAcuity™, Roche Digital LightCycler®, and Bio-Rad QX100™, QX200™, and QX600™ dPCR platforms.

Do you offer technical support?

Yes! GT Molecular’s technical support scientists are available from 8:00am to 5:00pm (MT) Monday through Friday to answer any questions regarding our products. Contact us at 1 970-498-1698 or through this form, and we’ll be happy to walk you through the workflow and answer any questions you may have.

Which targets are measured by the GT-Plex™ KRAS G12C Multiplex PCR Kit?

The GT-Plex™ KRAS G12C Multiplex PCR Kit covers the G12C mutation and the normal G12 allele in exon 2 of the KRAS gene.

What positive controls are used with the GT-Plex™ KRAS G12C Multiplex PCR Kit?

KRAS G12C and non-mutant KRAS are included as qualitative positive controls.

How soon after placing an order can I expect delivery of the kit?

Once an order is placed, most assay kits are shipped within 5 business days on ice via FedEx Priority Overnight.

What sample types can be used with the GT-Plex™ KRAS G12C Multiplex PCR Kit?

Our oncology research kits are suitable for use with formalin-fixed paraffin-embedded (FFPE) tissues, liquid biopsy, fresh/frozen tissue, cell-free DNA (cfDNA), circulating tumor DNA (ctDNA), and low-yield samples. Our dPCR assays also tolerate samples that contain PCR inhibitors.