dPCR NPM1 Mutation Detection Assay Kit for the Bio-Rad QX200™ Droplet Digital™ PCR System

(Cat# 100400)

The dPCR NPM1 Mutation Detection Assay Kit for the Bio-Rad QX200™ Droplet Digital™ PCR System is a Research Use Only molecular reagent kit containing all primers, probes, and controls for the detection of DNA with NPM1 mutations common in acute myeloid leukemia (AML). The assay is designed to detect over 40 mutation types, including Type A, B, and D mutations.

The assay solution contains a FAM probe which targets non-mutated sequence at NPM1-W288 where common insertions occur and a HEX probe targeting non-mutated sequence within the same amplicon. The presence of a short nucleotide insertion in the region of the FAM binding sequence results in a HEX-only positive signal (from the absence of FAM signal), whereas the presence of wild-type NPM1 results in a FAM+HEX double positive signal. The assay was validated for the detection of the Type A NPM1 insertion (c.863insTCTG) and can also presumably detect, but not discriminate between, Type B, D, and other insertions occurring between c.863 and c.866. Rare NPM1 insertion types occurring between c.867 to c.875 can also presumably be detected with the assay, resulting in a FAM-only positive signal (from the loss of HEX signal. This kit includes one preformulated primer probe solution and qualitative NPM1 wild-type and Type A mutant controls.